Following her departure from 24, Aylesworth has remained active in both television and movies as well as theatre. Her first leading role in a feature film was in the independent, romantic drama Crazylove where she worked alongside Bruno Campos and former 24 costar Paul Schulze. Crazylove tells the story of Letty Mayer, a school teacher who suffers a nervous breakdown and while institutionalized, meets Michael (Campos) and the two begin a dangerous and unpredictable romance. She partnered with her former 24 costar Xander Berkeley in the Sci Fi Channel made-for-television movie, Magma: Volcanic Disaster. In a guest start role on the short-lived series Conviction, she played a renowned attorney who killed her husband and then defended herself at the murder trial. When the cast was selected for the series 3 lbs, Aylesworth was chosen for the role of Dr. Adrienne Holland, but the pilot was rejected by CBS. The following season, CBS decided to buy the series for a late fall 2006 premiere, but the lead roles were recast, including Aylesworth's. It is unknown whether she declined the 3 lbs role or she was not offered it again.